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KMID : 0355519880120020098
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Korean Journal of Oral Biology
1988 Volume.12 No. 2 p.98 ~ p.108
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A STUDY ON THE ADHESION OF ORAL STREPTOCOCCI TO PROTEIN-COATED HYDROXYAPATITE BEAD
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Abstract
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Effect of human and animal sera and a serum protein on the adherence of oral streptococci to hydroxyapatite bead was investigated by standard adherence assay procedure. Sucrose effect on bacterial adherence to sa.liva-coated HA (SHA) bead was also studied. It was examined whether bead-adhered bacterial cells remained attached to or detach from bead in scintillant, because sample self-absorption could take place.
Animal sera and BSA decreased bacterial adherence to HA. BSA concentration as high as 10mg/rnl inhibited the adherence up to 87%.
When HA bead pre-coated with BSA was treated with saliva, no difference in the ex-tent of adherence was observed as compared to the control bead treated only with saliva. S.utans strain 10449 among several other strains and a strain of S. sanguis showed the fastest agglutination upon addition of sucrose. S. mutans cells exposed to sucrose to permit extracellular polysaccharide synthesis before or during adherence attached in fewer numbers to both HA and SHA than control organisms. This may be due to a formation of large-sized aggregates. HA bead-adhered bacterial cells in scintillation fluid remained attached during scintillation counting, thus raising a, possibility of sample self- absorption in radioisotope counting rate.
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